Supplementary MaterialsAdditional file 1 Physique showing specificity for ALDH1A1 and ALDH1A3 antibodies used in immunostainings

Supplementary MaterialsAdditional file 1 Physique showing specificity for ALDH1A1 and ALDH1A3 antibodies used in immunostainings. ALDEC primary human mammary epithelial cells separated by with ACS were immunostained for ER (FITC) and reanalyzed with flow cytometry. ALDEC cells contained 27.8% ER+ cells CA-4948 (left panel), whereas ALDE+ cells did not express ER above background level (right …
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Supplementary Materialspharmaceutics-12-00816-s001

Supplementary Materialspharmaceutics-12-00816-s001. C, and the proteins concentration was dependant on Bradford assay (5000001, Bio-Rad, Hercules, CA, USA). For co-immunoprecipitation (Co-IP), the supernatant was treated with 1 g/mL from the indicated antibodies RhoA at 4 C for 16 h with soft shaking, accompanied by incubation with agarose G proteins beads (Santa Cruz, Dallas, TX, USA) for …
Continue reading Supplementary Materialspharmaceutics-12-00816-s001